休克饮抗肠菌转位的实验研究

Experimental Study on Mechanism or Xiukeyin in Anti-Bacterial Translocation from Gut

目的:观察休克饮的抗肠菌转位作用，为临床危重病患者多脏器衰竭（ＭＯＦ）的中医治疗提供依据。方法：根据严重创伤如休克等引起肠菌转位及肠源性脓毒症继而导致ＭＯＦ的病理生理学基础，建立出血性休克诱发肠菌转位的大鼠模型，用以确定温脾汤加减组成的休克饮对肠菌转位的影响。结果：以休克饮灌胃的休克组大鼠肠道细菌转位至肠系膜淋巴结、肝、脾的发生率（３／１５）比未灌胃休克组大鼠的发生率（１１／１３）明显降低（Ｐ＜０．０１）；但两组大鼠肠腔内生长细菌的种类和数量无明显差异（Ｐ＞ ０． ０５）；组织学检查所见，休克组大鼠肠粘膜水肿比休克饮治疗休克组明显。结论：休克饮可抑制出血性休克诱发的肠菌转位，其机理可能是保护肠粘膜，而对肠腔内细菌的生长无明显影响。

To provide a TCM therapeutic base for treatment of critical multiple organ failure (MOF) patients clinically, the mechanism of Xiukeyin (XKY, Shock Beverage) in anti-bacterial translocation from gut in rats was studied. Metals: Based on the pathophysiology of MOF following severe injury, such as shock caused enterogenoussepsis and bacterial translocation from gut, a hemorrhagic shock model of rat was established and used to determine the effect of XKY on anti-bacterial translocation. XKY was formulated by modifying Wenpi Decoction. Results: The inddence of intestinal bacterial translocation to mesenteric lymph nodes, liver and spleen was lower in the model rats infused via gastrogavage with XKY (3/15) than that in the noninfused rats (11/13) (P<0.01). The amount and species of intestinal flora in XKY infused or noninfused rats were not different statistically (P> 0.05). Histological examination showed that the intestinal edema was severer in the model group than that in the XKY treated group. Conclusions: XKY could inhibit the shock induced enterogenous bacterial translocation, the mechanism might be due to its protective action on intestinal mucosa. XKY showed no effect on the growth of intestinal bacteria.