摘要
目的:探讨神经元凋亡与颞叶癫癎患者海马硬化的关系。方法:取15例颞叶癫癎患者手术切除标本(癫癎组)和5例无癫癎发作的患者正常脑组织标本(对照组),用原位末端标记(TUNEL)方法检测神经元凋亡,免疫组化染色检测细胞凋亡相关基因表达产物Bcl-2、Bax、Caspase-3蛋白的表达。结果:癫组和对照组的TUNEL阳性细胞平均百分率分别为(52.2±3.2)%、(4.0±1.8)%,差异有统计学意义(P<0.05)。免疫组化染色结果表明,对照组患者脑组织内Bcl-2蛋白无表达,癫癎组患者脑内Bcl-2蛋白表达明显增强(P<0.05);Bax蛋白在癫癎组与对照组中均微弱表达,差异无统计学意义;Caspase-3在对照组中有轻微表达,在癫癎组表达明显增强,差异有统计学意义(P<0.05)。结论:神经元凋亡部分参与癫癎患者海马硬化的形成,Bcl-2、Bax和Caspase-3蛋白在这一过程中可能发挥作用。
Objective: To explore the relationship between neuronal apoptosis and hippocampus sclerosis of patients with temporal lobe epilepsy. Methods: The brain tissue samples were obtained from 15 patients with temporal lobe epilepsy and 5 normal controls during operation. Neuronal apoptosis was detected hy TUNEL method, and the expressions of Bcl-2, Bax and Capase-3 were determined by immunohistochemistry. Results: TUNEL positive cells (52.2%)in the epileptic brain were significantly numerous than the normal brain (4. 0%) (P〈0.05). When compared with normal brain, Bcl-2 immunoreactivity was upregulated significantly in the epilepsy group (P〈 0.05). Similarly, caspase-3 expression was significantly up-regulated compared with that in control (P〈0.05), but Bax immunoreactivity was weakly detected in both the normal and epileptic brain and showed no significant difference between them. Conclusion: Neuronal apoptosis is involved in hippocampus sclerosis of human brain with epilepsy. Apoptosis related proteins such as Bcl-2, Bax and Caspase-3 protein may play a active role in this process.
出处
《神经损伤与功能重建》
2009年第3期170-172,共3页
Neural Injury and Functional Reconstruction