摘要
目的:探讨神经元凋亡与颞叶癫癎患者海马硬化的关系。方法:取15例颞叶癫癎患者手术切除标本(癫癎组)和5例无癫癎发作的患者正常脑组织标本(对照组),用原位末端标记(TUNEL)方法检测神经元凋亡,免疫组化染色检测细胞凋亡相关基因表达产物Bcl-2、Bax、Caspase-3蛋白的表达。结果:癫组和对照组的TUNEL阳性细胞平均百分率分别为(52.2±3.2)%、(4.0±1.8)%,差异有统计学意义(P<0.05)。免疫组化染色结果表明,对照组患者脑组织内Bcl-2蛋白无表达,癫癎组患者脑内Bcl-2蛋白表达明显增强(P<0.05);Bax蛋白在癫癎组与对照组中均微弱表达,差异无统计学意义;Caspase-3在对照组中有轻微表达,在癫癎组表达明显增强,差异有统计学意义(P<0.05)。结论:神经元凋亡部分参与癫癎患者海马硬化的形成,Bcl-2、Bax和Caspase-3蛋白在这一过程中可能发挥作用。
Objective： To explore the relationship between neuronal apoptosis and hippocampus sclerosis of patients with temporal lobe epilepsy. Methods： The brain tissue samples were obtained from 15 patients with temporal lobe epilepsy and 5 normal controls during operation. Neuronal apoptosis was detected hy TUNEL method, and the expressions of Bcl-2, Bax and Capase-3 were determined by immunohistochemistry. Results： TUNEL positive cells （52.2%）in the epileptic brain were significantly numerous than the normal brain （4. 0%） （P〈0.05）. When compared with normal brain, Bcl-2 immunoreactivity was upregulated significantly in the epilepsy group （P〈 0.05）. Similarly, caspase-3 expression was significantly up-regulated compared with that in control （P〈0.05）, but Bax immunoreactivity was weakly detected in both the normal and epileptic brain and showed no significant difference between them. Conclusion： Neuronal apoptosis is involved in hippocampus sclerosis of human brain with epilepsy. Apoptosis related proteins such as Bcl-2, Bax and Caspase-3 protein may play a active role in this process.
出处
《神经损伤与功能重建》
2009年第3期170-172,共3页
Neural Injury and Functional Reconstruction
