罗非鱼蛋白自溶水解条件优化

Autolysis of Tilapia Proteins and Optimization of the Conditions for It

以奥尼罗非鱼肉为原料进行自溶水解实验,探讨了水解液中的水解度(DH)、可溶性蛋白及可溶性固形变化,并研究了pH值、温度、时间、料液比对奥尼罗非鱼肉自溶水解效果的影响.通过单因素和L9(34)正交试验,得出了奥尼罗非鱼肉自溶的最佳工艺参数:料液比1∶2(m/v),温度45℃,pH值7.5,时间6 h.在此条件下,氨基态氮含量及可溶性固形物含量分别为0.745 4 g/L、2.18%、水解度为8.28%,蛋白质回收率达到89.07%,挥发性盐基氮为10.5 mg/kg.

In an autolysis experiment with the flesh of Tilapia as the main raw material, DH, soluble protein and soluble solids were used as indicators to determine the effects of pH, temperature, hydrolysis time and solid-liquid ratio on hydrolysis. Using single-factor and L9 （3^4） orthogonal experiments, the conditions for Tilapia autolysis were optimized, the best technological parameters being solid-liquid ratio 1 ： 2 （m/v）, reaction temperature 45℃, pH 7.5 and reaction time 6 h. Under these conditions, amino nitrogen and soluble solids contents were 0. 745 4 g/L and 2.18%, respectively, DH was 8. 28%, protein recovery rate was 89.07% and total volatile basic nitrogen （TVBN） was 10.5 rag/100 g.