摘要
目的比较慢性和急性染镉所致肾损伤的差异。方法给小鼠多次注射CdCl2(剂量:镉0.8~1.2mg/kg,每周6次,共6周),或一次给予大量注射镉金属硫蛋白复合物(CdMT,剂量:镉0.2~0.4mg/kg),然后比较其肾损伤。结果小鼠一次性大量注射CdMT主要产生肾脏近曲小管的坏死,虽然其肾镉含量仅为8μg/g,但尿蛋白、尿糖、尿酶活力和血尿素氮含量均明显增高。与此相比,慢性染镉的小鼠肾镉含量高达135μg/g,但仅有轻度近曲小管坏死及浊肿,上述尿、血指标仅略为增高。除近曲小管损伤外,慢性染镉还造成小鼠肾小球损伤,间质炎症,肾小管细胞凋亡、萎缩及再生。小鼠CdMT在慢性染镉后升高100倍,达450μg/g,但急性染镉仅略升高到25μg/g。结论用注射CdMT的急性染镉法来研究慢性染镉所致的肾损伤有一定的局限性。
In order to study the difference of renal injury between chronic and acute cadmium exposure,mice were given daily sc injections of CdCl2(0.8 ̄1.2 mg Cd/kg,six times a week,for 6 weeks),or a single ip injection of cadmiummetallothionein complex(CdMT,0.2 ̄0.4 mg Cd/kg) to induce renal injury.Multiple injections of CdCl2 resulted in renal Cd load of 135 μg/g,along with a 100fold increase in renal MT.By contrast,renal Cd load in acute CdMTtreated mice was only 8 μg/g,with a mild induction of MT(5fold).In spite of lower Cd in kidneys,acute CdMT produced severe tubular necrosis,with remarkable proteinuria,glucosuria,enzymuria and elevated blood urea nitrogen.However,chronic CdCl2 produced tubular cell apoptosis as a feature of the lesion,and renal injury was mild.Besides,glomerular lesions and interstitial nephritis were also apparent following chronic CdCl2 administration.These results suggest that acute CdMT injection may not be an appropriate model to study chronic Cd nephropathy.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1998年第1期9-11,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
镉
金属硫蛋白
复合物
肾损伤
细胞凋亡
Cadmium Cadmiummetallothionein complex(CdMT) Renal injury Apoptosis