镉对小鼠肢芽细胞增殖细胞核抗原和c-myc基因表达的影响

Effects of cadmium on the expression of PCNA and cmyc in mouse embryo limb bud cell

目的探讨镉对胚胎肢芽细胞增殖抑制的机制。方法运用免疫组化ＳＰ法结合图像分析技术观察了ＣｄＣｌ２对增殖细胞核抗原（ＰＣＮＡ）和ｃ－ｍｙｃ基因表达的影响。结果ＰＣＮＡ和ｃ－ｍｙｃ基因在正常增殖的胚胎肢芽细胞中均呈强阳性免疫反应；０．１～０．８μｇ／ｍｌＣｄＣｌ２对细胞中ＰＣＮＡ和ｃ－ｍｙｃ的表达均有明显的抑制作用，并呈现明显的剂量－效应关系。结论ＣｄＣｌ２可通过抑制ＰＣＮＡ和ｃ－ｍｙｃ的表达而对胚胎细胞增殖产生抑制作用。

The present study is to investigate the mechanism of cessation of proliferation of mouse embryo limb bud cell exposed to cadmium,in which immunohistochemical SP method with assistance of image analysis was conducted to observe the effects of cadmium on the expression of proliferating cell nuclear antigen[WT5”,6BZ](PCNA) and cmyc protooncogene in mouse embryo limb bud cell.The results showed that there was strong positive expression of[WT5”,6BZ] PCNA as well as cmyc in normal proliferating mouse embryo limb bud cell;however,[WT5”,6BZ]CdCl2 at the dosage of[WT5”,6BZ] 0.1￣0.8 μg/ml could significantly reduce the expression of the two genes and obvious dosedependent effect was also observed.It is suggested that Cd could inhibit mouse embryo limb bud cell proliferation by decreasing the expression of PCNA and cmyc.