镉对大鼠睾丸间质细胞脂质过氧化的影响

Effects of cadmium on lipid peroxidation of Leydig cells of rat testis in vitro

目的观察镉对离体睾丸间质细胞的毒性作用。方法测定不同ＣｄＣｌ２染毒浓度下（含镉０、１０、２０和４０μｍｏｌ／Ｌ）间质细胞的脂质过氧化水平。结果染毒２４小时后，染毒组的细胞存活率显著低于对照组（Ｐ＜０．０１），且存在剂量－效应关系；随染毒浓度的增高，丙二醛（ＭＤＡ）含量和谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活力显著增高（Ｐ＜０．０５），而超氧化物歧化酶（ＳＯＤ）活力则显著下降（Ｐ＜０．０１）。相关分析结果显示：ＳＯＤ与ＭＤＡ呈负相关、与ＧＳＨ－Ｐｘ呈显著负相关（ｒ＝－０．４３３９，Ｐ＜０．０５）；ＭＤＡ与ＧＳＨ－Ｐｘ呈显著正相关（ｒ＝０．４３２９，Ｐ＜０．０５），表明镉可使睾丸间质细胞的脂质过氧化作用增强、抗氧化能力减弱。结论镉对睾丸间质细胞具有直接毒性作用。

In order to study the toxic effects of cadmium on Leydig cells of rat testis,lipid peroxidation levels of Leydig cells exposed to different concentration of cadmium(i.e.0,10,20 and 40 μmol Cd/L) in vitro were determined.The results showed that twenty-four hours after exposed to cadmium,the cells' survival rate in the exposed groups was significantly lower than that in the control group(P<0.01).It was found that there were dose effect relationships between the cell survival rate and cadmium exposure.With increased cadmium exposure dose,the contents of MDA and GSHPx activity in the exposed groups were significantly higher than those in the control(P<0.05),but SOD activity was inverse(P<0.01).Correlation analysis showed that there were negative correlation between SOD and MDA or GSHPx(r=-0.433 9,P<0.05),and positive correlation between MDA and GSHPx(r=0.432 9,P<0.05).It is indicated that Cd could increase lipid peroxidation level and reduce antioxidation ability of Leydig cells from testis in rat,suggesting that cadmium may have direct toxic on them.