IGF2和H19基因与儿童胚细胞恶性肿瘤的关系

Relationship of IGF2 and H19 Genes in Children with Embryogenic Cancers

目的：探讨儿童胚细胞起源的恶性肿瘤的发生与ＩＧＦ２和Ｈ１９基因胚胎印迹缺失（ＬＯＩ）的关系及肿瘤时这两个基因之间的协同关系。方法：通过ＰＣＲ－ＲＦＬＰ法和Ｎｏｒｔｈｅｒｎｂｌｏｔ法检测包括６例肾母细胞瘤、２例神经母细胞瘤和２例内胚窦瘤在内的１０例儿童胚细胞起源的恶性肿瘤中ＩＧＦ２和Ｈ１９基因的ＬＯＩ及Ｈ１９基因的ｍＲＮＡ表达。结果：①三种肿瘤中均可检测到频发的ＩＧＦ２基因的ＬＯＩ，Ｈ１９基因的ＬＯＩ相对少见；②肾母细胞瘤中发生ＩＧＦ２基因ＬＯＩ的病例全部伴有Ｈ１９基因ｍＲＮＡ的低水平表达。结论：ＩＧＦ２基因的ＬＯＩ与儿童胚细胞起源的恶性肿瘤的发生有密切关系，而这两个基因的相互协同作用可能是肾母细胞瘤发病的重要因素之一。

Objective: To investigate the relationship of the loss of imprinting (LOI) of insulinlike growth factor2 (IGF2) and H19 gene with tumorigenisis of childhood embryogenic cancers and their coordinative effects. Methods: LOI of IGF2 and H19 genes, and mRNA expression of H19 gene were examined on 10 cases of embryogenic cancers including Wilms tumors (6), Neuroblastomas (2) and Yolk sac carcinomas (2) by PCRRFLP and Northern blot methods. Results: LOI of IGF2 always appeared in the above tumors while LOI of H19 was less frequent, and LOI of IGF2 was detected in association with reduced H19 mRNA expression in Wilms tumor. Conclusions: LOI of IGF2 may be associated with tumorigenesis of embryogenic cancers and IGF2 worked in coordination with H19 genes may play an important role in the incidence of Wilms tumor.