喉癌患者颈淋巴结中溶菌酶抗肿瘤作用的研究

The study on the anti-tumor function of lysozyme in the regional lymph nodes of laryngocarcinoma patients

为探讨喉癌患者颈淋巴结中溶菌酶（LZM）的抗肿瘤作用，采用免疫组化技术对喉癌患者颈淋巴结石蜡切片中的LZM分布进行观测；采用细胞培养技术，以喉癌患者颈淋巴结中提取的Mφ作效应细胞，自体癌细胞作靶细胞，混合培养，ConA刺激原作对照，测定Mφ杀伤能力及LZM含量。结果表明：癌转移阴性淋巴结石蜡切片中LZM阳性巨噬细胞（LPM）数目高于癌转移阳性淋巴结（P＜0．01）；ConA刺激后，Mφ释放的LZM含量及杀伤能力均明显提高（P＜0．01）；癌转移阴性淋巴结的Mφ释放LZM含量及杀伤自体喉癌细胞能力均高于癌转移阳性淋巴结（P＜0．05）；LZM含量与喉癌细胞死亡率呈正的线性相关（r＝0．94，P＜0．01）。提示，喉癌患者颈淋巴结中LZM在抗肿瘤免疫中起重要作用，能提高Mφ对自体喉癌细胞的杀伤能力，并促进喉癌细胞死亡。

The purpose of this study was to discuss the anti-tumor function of lysozwne(LZM) to largngocarcinoma. The LZM distribution of regional lymph nodes incised from laryngo-carcinorna patients in paraffin sections was observed by immunohistochemical technique. By thecell culture technique, autologUs laryngoCarcinoma cells were used as target cells to evaluate thetumoridical activity of Mg which were isolated from the reglonal lymph nodes of laryngoarcinomapatients. At the same time,ConA was added as control group. The supernatant of cell culture wascollected after 24 hours to detect LZM content. The results indicated that the number of LPM innon-metastatic lpoph nodes was significantly more than in metastatic 1yInPh nodes (P<0.01 ).The Mφ isolated from patients regional lymph nodes released LZM content and it's tumoridicalactivity to autologus tumor cells were sighficantly increased after adding ConA (P <0.01). TheLZM releasing content and tumoridical activity of Mφ in non-metastatic lyrnph nodes were muchmore than metastatic lymph nodes (P<0.05). The LZM content and tumoridical activity of Mφwere analyzed with linearity relapsing statistics, it was positive linearity relation (r = 0. 94, P<0.01). All above suggests that the LZM of laryngocarcinoma patients is closely related to the anti-tumor function, it improves the tumoridical activity of MN to autologus laryngocarcinoma cellsand accelerates the death of laryngocarcinoma cells.