具有血管紧张素转化酶抑制活性益生菌筛选的研究

Screen of probiotics having angiotensin converting enzyme-inhibition activity

经体外血管紧张素转换酶(ACE)抑制活性实验,从保存的40株菌中筛选到7株具有降压活性的益生菌,其中菌株DM8319粗提样品经初步凝胶层析纯化后其IC50值从0.038 mg/ml降到0.016 mg/ml,达到了初步分离效果。后依据益生菌筛选标准,对上述菌株抗氧化性、耐酸、耐胆盐、抑菌和耐药性能进行考察。结果显示,菌株DM84013最适用于益生菌降压产品的研究,经初步鉴定该菌种为长双歧杆菌。

In vitro experiment was conducted on the inhibition of angiotensin converting enzyme （ACE）. The result indicated that, among the 7 strains with blood pressure-reducing activity which were screened out of 40 probiotics stored in our lab ,the IC50 value of the crude sample of strain DM8319 decreased from 0. 038 mg/ml to 0.016 mg/ml based upon preliminary Gel chromatography purification. The antioxidation property, resistances to acids, bile salt and antibiotics as well as the inhibition to bacteria of the 7 probiotics were determined according to the sceening standard of Prebiotics, and the results showed that DM8do13 ,which had been identified preliminarily as B. langum,was the best to be used in research of probiotic-based antihypertensive products.