临床标本中酵母样真菌的分离鉴定和药敏结果分析

The separation and identification of yeast-like-fungi from clinical specimens and the analysis of drug susceptibility

目的调查目前临床标本中酵母样真菌感染现状及耐药性情况。方法常规分离临床标本中的酵母样真菌,用科玛嘉显色培养基及法国生物-梅里埃API 20C AUX鉴定系统鉴定菌种,并用K-B法进行药敏试验。结果研究期间共分离到193例有临床意义的酵母样真菌,检出率较高的依次为白假丝酵母菌(62.7%),热带假丝酵母菌(20.2%),光滑球拟酵母菌(9.3%)。在6种抗真菌药中,对伊曲康唑、氟康唑的耐药率增高。结论近年来由酵母样真菌感染引起的疾病及其对常用抗真菌药物的耐药率有明显增加,应加强真菌的检测和耐药性监测工作,以指导临床合理使用药物。

Objective To investigate the characteristics of yeast-like fungi infection and antifungal resistance at present. Method The yeast-like fungi in clinical specimens were isolated by conventional method and identified by CHRO Magar Candida medium and API 20C AUX identification system. The antifungal sensitivity was detected with the K-B mothod. Result A total of 193 yeast-like fungi were detected, with the dominant species being C. a/b/cans （62.7%）, C. tropicalis （20.2%） and C. glabrate （9.3%）. Among 6 antifungal agents, the resistance against fluconazole and itraconazole were increasing. Conclusion The infectious diseases induced by yeast-like fungi had increased these years, and the re- sistance of those fungi tends to become more serious. The detection of fungi and antifungal sensitivity test should be strengthened in order to guide clinical selection of the reasonable antibiotic.