球红假单胞菌的微波诱变及对煤炭转化的影响

Influence on Microwave Mutagenesis of Spheroid Rhodopseudomonas and Coal Conversion

通过对球红假单胞菌进行微波辐射诱变处理,选育出一株对低阶煤具有较强降解转化能力的突变体,对义马长焰煤的生物降解转化率从7.82%提高到35.31%,对硝酸处理过的义马长焰煤生物降解转化率从41.72%提高到60.34%。该试验研究中还应用傅里叶变化红外线光谱(FTIR)对煤炭生物降解转化产物进行了测试分析。结果表明,煤炭发生生物降解转化的原因是由于细菌能释放出对煤炭具有降解转化作用的酶,使其芳香聚合度和分子量有很大程度的降低,官能团含量也发生了变化,煤样中芳香结构中大分子物质的支链、侧链断裂,变成羧基类、羟基类和醇基类等易溶于水的小分子物质。

A strong low rank coal degrading mutant was selected by microwave irradiation treatment of Rhodopseudomonas spheroides, which is capable of degrading low rank coal, Its biodegradation of Yima long flame coal increases from 7.82% to 35.31% and long flame coal treated by nitric acid infusion was increases from 41.72% to 60. 34%. The degradation products were analyzed using Fourier Transform Infrared Spectroscopy （FTIR） , and the results show that the cause of coal to degradation is the function of enzyme released from the Rhodopseudomonas spheroides, the degree of the polymerization of the aromatic nucleus and the molecular weights of products as well are decreased obviously, and remarkable changes also take place in the content of functional groups compared with the coal, and the function of enzyme could break the branch chain and side chain of the big molecules in aroma structure of coal sample and then turn to small molecules, such as carboxy, hydroxy and aleoho substances easy to dissolve in water.