小儿心肌疾病抗肌球蛋白抗体和粒细胞-巨噬细胞集落刺激因子的研究

The clinical study of anti-myosin and GM-CSF in children with viral myocarditis and dilated cardiomyopathy

为探讨病毒性心肌炎（VMC）和扩张型心肌病（DCM）发病的自身免疫机制，采用酶联免疫吸附和放射免疫技术检测30例VMC、14例DCM患儿血浆中抗肌球蛋白抗体和粒细胞-巨噬细胞集落刺激因子（GM－CSF）。结果：VMC和DCM患儿抗肌球蛋白抗体和GM－CSF的阳性率均为54．5％（24／44），而正常对照组分别为4．0％（1／25）和8．0％（2／25）（均P＜0．01），且抗肌球蛋白抗体与GM－CSF的血浆水平有正相关性（r＝0．3583，P＜0．05）。提示：抗肌球蛋白抗体和GM－CSF均参与了VMC和DCM的发病，与心肌的自身免疫损伤过程有关。

In order to explore the autoimmune pathogenic mechanisms in viral myocarditis (VMC) and dilated cardiomyopathy (DCM), the plasma autoantibody against myocardial myosin and granulocyte-macrophage colonystimulating factor (GM-CSF) in children(30 with VMC, 14 with DCM ) were studied with enzyme-linked immunosorbent assay and radioimmunoassay. Results:The positive rates of anti-myosin and GM-CSF in children suffered from viral myocarditis and dilated cardiomyopathy were same as 54. 5 % (24/44),while those of normal control group were 4. 0% (1/25 )and 8. 0 % (2/25 ) respectively (both are P <0.01),and there was significant correlation between the plasma levels of anti-myosin and GM-CSF(r =0. 358 3). Thus it is indicated that anti-myosin and GM-CSF both participate in the pathogenesis of VMC and DCM,and related with the process of the autoimmune injury of myocardium.