应用生物素-链霉亲和素的酶联免疫吸附法检测谷物中的玉米赤霉烯酮

Detection on Zearalenone in Grains by Biotin-streptavidin Enzyme-linked Immunosorbent Assay

基于生物素-链霉亲和素的酶联免疫吸附法(ELISA),建立了玉米赤霉烯酮(ZEN)的ZEN-ELISA检测方法。该方法的检测灵敏度为0.8 ng/ml,批内、批间变异系数分别为5.1%和8.6%,平均加样回收率为91.2%;抗ZEN单克隆抗体与玉米赤霉醇的交叉反应度为12.3%,而其与赭曲霉素A、脱氧雪腐镰刀菌烯醇、黄曲霉素B1无交叉反应;相关试剂在4℃存180 d后各检测参数基本无变化;该方法与商品ELISA试剂盒对同一样品ZEN测定结果的相关系数为0.943 2。说明该方法的特异性和稳定性较好,测定结果准确,可用于ZEN的大量筛查。

Based on biotin-streptavidin enzyme-linked immunosorbent assay（ELLISA）, the detection method on zearalenone ZEN-ELISA was estab- lished. The detection sensitivity of this method was 0.8 ng/ml, and the variation coefficients of intra-assay and inter-assay were 5.1% and 8.6 % resp., and the average recovery was 91.2%. The cross reaction rate of monoclonal antibody against ZEN and zearalanol was 12.3%, and there were no reaction between moneelonal antibody against ZEN and ochratoxin A, deoxynivalenol, aflatoxin B1. When related reagents were stored at 4 ℃ for 180 d, the detection parameters almost invariant. The correlation coefficient of the detection results of ZEN-ELISA method and commercial ELISA kit on the same sample was 0.943 2. Which indicated that the specific and stability of ZEN-ELISA method were better, and its detection results was accurate, so it could be used to largely screen ZEN.