摘要
[目的]对穿心莲中穿心莲内酯的提取工艺进行研究。[方法]首先对不同提取溶剂进行考察,然后采用正交试验、单因素试验进行条件优化。[结果]溶剂考察结果为以碱水、醇及水为溶剂时提取率分别为5.25‰、3.95‰、3.08‰;碱水提取时碱浓度为0.01%穿心莲内酯提取率最高,平均为5.64‰;醇提正交试验得出最佳工艺为10倍量85%乙醇提取2次,平均提取率为4.80‰。[结论]从提取率及经济性考虑,碱水溶液提取比乙醇溶液提取较佳。
[Objective]The research aimed to study the optimum extraction process of andrographolide from Andrographis paniculata (Burm. f. )Nees. [Method] Firstly, different extraction solvent were studied to choose the best extract solvent, then two kind solvents, ethanol and alkaline extraction pro- cesses were optimized by the orthogonal experiment and the single factor experiment respectively. [Result] Solvent extraction study showed the extraction ratio of andrographolide by alkali, ethanol, water was 5.25‰, 3.95‰, 3.08‰ respectively. Results of different concentration of sodium carbonate water solution extraction experiment showed: 0.01% alkaline extraction could get the maximam extraction rate, the average extraction rate is 5.64‰.The best ethanol process was: adding 10 times of 85‰ethanol, refluxing for 2 times, the average extraction rate was 4.80‰. [Conclusion] Compared these two extract methods, the sodium carbonate extract was better.
出处
《安徽农业科学》
CAS
北大核心
2009年第15期6988-6989,共2页
Journal of Anhui Agricultural Sciences