利用电极盘进行猪卵母细胞电激活

Electrical Activation of Porcine Oocytes with Electropolar Plates

[目的]研究不同电激活参数以及电脉冲联合6-DMAP对猪卵母细胞孤雌激活效果的影响。[方法]使用电极盘，测定不同场强（1．1、1．3、1．5、1．7、1．9kV／cm），不同脉冲时程（30、50、70、90、110ps），1次脉冲下猪卵母细胞囊胚率的变化。[结果]结果表明，脉冲强度以1．5和1．7kV／cm效果最好，在80胂时其猪卵母细胞囊胚率分别达到（22．35±5．16）％和（20．59±9．41）％；脉冲强度1．5kWcm，1次电脉冲后4h联合6-DMAP激活卵母细胞，当脉冲时程达到70和90μs时，囊胚率分别达到（21．65±9．95）％和（23．10±16．27）％。[结论]在试验条件下，使用电极盘的最适电激活参数为脉冲强度1．5-1．7kV／cm，脉冲时长80μs，1次脉冲电激活。

[Objective]The research aimed to study the effects of different field strength and electricity combined with 6-dimethylaminopurine （6-DMAP） on the parthenogenetic development of porcine oocytes using electropolar plate. [ Method] Porcine oocytes blastacyst rotes were rneasured under the situations of different field strength （ 1. 1, 1.3,1.5,1.7,1.9 kV/cm ）, and different pulse duration （ 30,50,70,90,1 10μs ）, one times of pulse with electropolar plate. [Result]The results showed that, 1.5 and 1.7 kV/cm field strength were better, and the porcine oocytes blastecyst rates was （22.35 ± 5.16）% and （20.59± 9.41 ）% with duration of 80/is. Treated with I. 7 kV/em field strength, one times of pulse arid combined with 6-DMAP 4 h later, the porcine cocytes blastocyst rates was （21.65 ± 9.95） % and（23.10 ± 16.27） % when pulse duration was 70 and 90μs. [ Conclusion] In conclusion, the best electrical parameters for porcine oecyte parthenogenesis was 1.5 - 1.7 kV/cm with one 80μs when using etectropolar plate.