黔产野生山楂中齐墩果酸的含量测定研究

Determination of Oleanolic Acid in Guizhou Wild-hawthorn by HPLC

[目的]探寻黔产野生山楂中齐墩果酸含量的测定方法。[方法]采用HPLC法,DiamonsilTM(钻石)C18色谱柱;以甲醇-0.8%醋酸铵(80∶20)为流动相;流速为1.0 ml/min;柱温30℃;检测波长210 nm。[结果]方法线性关系良好,平均加样回收率99.92%(RSD=0.144 0%)。[结论]HPLC法简便快速、结果准确,可以作为测定黔产野生山楂中齐墩果酸含量的测定方法。

[Objective]The research aimed to establish the determination method of oleanolic acid in Guizhou wild-hawthorn. [Method] Dimuonsil^TM C18 column was used and methanol-0.8 % ammonium acetate（80： 20 ） was used as a mobile phase, velocity of 1.0 ml/min, column temperature of 30℃. The detective wavelength was 210 nm. [ Result ]This method showed a good linear relationship. The average recoveries was 99.92%, RSD was 0.144%. [ Conclusion] This method is simple,rapid,accurate,and it can be_nsed as the quantitative analysis method for this preparation.