抗内毒素单克隆抗体在脓毒症治疗中的评价

Therapeutic effects of endotoxin-directed monoclonal antibody on sepsis

目的制做并判定抗内毒素单克隆抗体对脓毒症治疗是否有效。方法以E coli J5,S,minnesotaRa,Re之LPS制备单抗M_2B,CLP大鼠或大肠杆菌一次注射小鼠伤前给于M_2B。结果 M_2B未能改善其细菌攻击小鼠的存活。M_2B有效提高CLP大鼠存活并减低血内毒素水平、炎性细胞因子水平,保持较高组织ATP与GSH。结论抗内毒素单抗在脓毒症治疗中并非无效而是有限,关键在于病情和时机。在适当时机对经选择病人不失为一种有益防治措施。

Objective To prepare endotoxin-directed monoclonal antibody and evaluate its effectiveness in the treatment of sepsis. Methods Endotoxin-directed monoclonal antibody M_2B is prepared with LPS from E eoli J5 and S Minnesota Ra Re as immunogen. The rats receiving CLP or the mice given bolus injection of Ecoli were given M_2B prior to challenge. Results M_2B failed to prolong the survival time in the mice giv-en bolus injection of E coli. In the rats receiving CLP, M_2B significantly improved survival, decreased blood endotoxin level and inflammatory cytokine level, and maintained higher contents of tissue ATP and GSH. Conclasion Endotoxin-directed monoclonal antibody might be a useful adjunct in the prevention and treatment of sepsis.