摘要
目的:制备胶体金免疫层析试纸检测猪链球菌2型。方法:用柠檬酸盐还原法制备胶体金颗粒,标记猪链球菌2型多克隆抗体,通过免疫层析作用对猪链球菌2型进行检测,并对试纸条的敏感性、特异性、稳定性进行评价。结果:每毫升胶体金最佳抗体标记量为22μg/mL,最佳包被抗体浓度为2mg/mL,最佳BSA封闭浓度为1.5%,建立的胶体金免疫层析试纸条检出猪链球菌2型的下限为106CFU/mL,从检测到结果判断时间为5~15min,与其他常见致病菌及链球菌属中15个群无交叉反应。结论:获得了检测猪链球菌2型的胶体金免疫层析试纸,该法操作简便,灵敏度高,特异性强,可用于猪链球菌的快速初筛和检测。
Objective: To prepare colloidal gold dipstick for rapid detection of Streptococcus suis serotype 2. Methods: Colloidal gold was prepared by citrate-natrium deoxidize method and coupled with the antibody against Streptococcus suis serotype 2, and Streptococcus suis serotype 2 was detected by immunochromatography assay in this study. Besides its sensitivity, specificity and stability were studied. Results: Gold immunochromatography assay was developed for detection of Streptococcus suis serotype 2, the best compatible protein concentration was measured as 22 μg/mL, the best coating protein concentration was measured as 2 mg/mL and the best blocking concentration was 1.5% of BSA. The detection sensitivity was as low as 106 CFU/ml and the strip has no cross-action with other bacteria detected. It took only 5-15 min from detecting to judging the result. Conclusions: The colloidal gold dipstick was prepared successfully, which was feasible, convenient, quick, and effective for detection of Streptococcus suis serotype 2.
出处
《现代生物医学进展》
CAS
2009年第10期1881-1884,1887,共5页
Progress in Modern Biomedicine
关键词
猪链球菌
胶体金
免疫层析法
Streptococcus suis serotype 2
Colloidal gold
Immunochromatography