口腔角质形成细胞与成纤维细胞共培养对胶原代谢的影响

Coculture of oral keratinocytes and fibroblasts affect collagen metabolicsm

目的:了解口腔角质形成细胞与成纤维细胞共同培养对胶原代谢的影响。方法:对口腔粘膜角质形成细胞和成纤维细胞进行了共培养,检测其胶原分泌水平、基质金属蛋白酶的含量及活性、基质金属蛋白酶抑制剂的水平。结果:只有共同培养组才能检测到MMP-9;共同培养组的活化型MMP-2水平与单独培养组相比无显著差异。角质形成细胞与成纤维细胞共同培养组的TIMP-1水平及胶原水平均明显高于单独培养组。结论:口腔角质形成细胞与成纤维细胞共同培养可能主要通过改变基质金属蛋白酶抑制剂的水平而影响胶原代谢。

Objective： To explore how oral keratinocytes and fibroblasts coculture affects the collagen metabolicsm. Methods： Oral keratinocytes and fibroblasts were cocultured. The content and activity of matrix metalloproteinase, the tissue inhibitor of metalloproteinase level and the level of collagen were assessed. Results： MMP-9 was produced only by co-culture group, No significant difference was seen between the active form of MMP-2 in coculture group and that of the non-coculture group. TIMP-1 and collagen produced by coculture group was significant higher than that by the non-coculmre group. Conclusion TIMP-1 plays important role in the collagen increasing in oral keratinocytes and fibroblasts coculture group.