摘要
目的本实验以皮罗卡平(Pilocarpine)致痫大鼠作为研究模型,探讨了致痫后大鼠海马APLP1mRNA表达的变化。方法经腹腔注射氯化锂(3mmol/kg)加皮罗卡平(30mg/kg)后,大鼠出现癫痫持续状态。将大鼠APLP1基因3'端进行克隆,并利用半定量RT-PCR检测了致痫后6h,30h,7d,15d时大鼠海马APLP1基因mRNA表达的变化。结果大鼠APLP1基因3'端与小鼠和人类APLP1基因的同源性分别为97%和90%,其氨基酸序列与小鼠相同,但与人的APLP1有三个碱基存在差异。在致痫6h后,海马APLP1mRNA表达水平显著下降(P<0.05),并在致痫后30h呈极低表达(P<0.01),一直持续到致痫后15d。结论APLP1基因3'端高度保守,APLP1基因mRNA的表达持续保持低水平。
Objective To investigate the expression of amyloid beta precursor-like protein 1 （APLP 1） gene on the transcription level in hippocampus of pilocarpine-induced epileptic rats. Methods Epileptic rats were developed by LiCl （3 mmol/kg, i.p.） approximately 20 h prior to pilocarpine （30 mg/kg, i.p.） administration. The 3＇ end partial sequence of rat APLP1 gene was cloned, and the expression levels of APLP1 mRNA in hippocampus of epileptic rats at 6 h, 30 h, 7 d and 15 d were determined by semi-quantitative RT-PCR. Results The 3＇ end partial sequence of rat APLP1 gene shared a 97% homology with that of mice, and 90% with that of human. The APLP1 amino acid sequence of rat was identical with that of mouse, but was different from that of human in 3 residues. Moreover, pilocarpine induced a significant down-regulation ofAPLP1 mRNA expression at 6 h after epilepsy initiation （P 〈 0.05）, and at 30 h, this down-regulation became more dramatic （P 〈 0.01）, which lasted till day 15 （P 〈 0.01）. Conclusion The 3＇ end ofAPLP1 gene is highly conserved, and APLP1 mRNA expression is kept at low level in hippocampus of pilocarpine-induced epileptic rats.
基金
supported by the National Basic Research Development Program of China (No.2006CB504501)
National Key Laboratory of Modern Communication Foundation of China (9140C1101050701)
the National Natural Sciences Foundation of China (No.30525030)
