HPLC法测定药用百合中薯蓣皂苷元的含量

Determination of diosigenin contents in medicinal Bulbus Lilii with HPLC

目的用HPLC法测定不同来源百合中薯蓣皂苷元的含量。方法采用Phenomenex ODS(4.6 mm×250mm,5μm)色谱柱,乙腈-水梯度洗脱,流速为0.7 mL/min,进样量为20μL,检测波长为203 nm,柱温为30℃。结果薯蓣皂苷元的进样量在0.04～1.60μg范围内与峰面积呈良好的线性关系。平均回收率为98.2%,RSD为2.1%(n=6)。结论该法操作简便、准确、灵敏度高,可作为药用百合质量控制的定量方法。

Objective To determine the diosgenin contents in Bulbus lain from different areas with RP-HPLC. Methods The RP-HPLC were with＇4.6 mm×250 mm, 5μm phenomenex ODS column, mobile phase of acetonitrile-water, 0.7 mL/min flow rate, 20 μL sample injection, 203nm wavelength and 30 ℃ temperature. Results The best linear correlation was the sample injection in the range of 0.04-1.60 μg and peak area（n=6）; and the average recoveries of diosgenin was 98.2%（RSD=2.1%,n=6）. Conclusion The methods is simple and accurate with a good sensitivity, which can be used for quality control of the medicinal Bulbus Lilii.