种子引发对夏枯草种子抗旱性的影响

Effects of seed priming on drought tolerence in Prunella vulgaris

目的：探讨增强夏枯草种子抗旱性的有效途径。方法：以不同浓度的聚乙二醇（PEG）溶液模拟干旱胁迫；引发处理后种子置于25％PEG干旱胁迫下发芽。结果：随着PEG胁迫浓度增加，夏枯草种子发芽率、发芽指数、活力指数下降；经过20％～25％PEG，300～500mg·L^-1 GA3，1．60％～2．00％KNO3-KH2PO4引发处理，3个居群夏枯草种子在干旱胁迫下种子发芽率以及发芽指数显著提高；经过0．40％～1．20％NaCl引发处理，南京紫金山、安徽金寨夏枯草种子抗旱性显著增强，种子发芽率以及发芽指数显著提高；0．40％～2．00％NaCl引发处理，甘肃夏枯草种子发芽率、发芽指数降低。结论：适量浓度PEG，GA3，KNO3-KH2PO4引发，可以增强夏枯草种子萌发阶段抗旱能力。

Objective： To explore an effective way to increase drought tolerance of Prunella vulgaris seed. Method： The treatment of drought stress to P. vulgaris seeds was made by the different concentrations of PEG solutions. Primed seeds germinated under 25% PEG. Result： As concentrations of PEG increasing, seed germination percentage, germination index and vitality index reduced. Primed with 20%-25% PEG, 300-500 mg· L^-1 GA3 and 1.6%-2.0% KNO3-KH2PO4 could enhance three population seeds germination index and vitality index under drought stress. Treated with NaCl, seeds germination percentage and germination index of two population increased, which came from Nanjing Zijinshan and Anhui Jinzhai, respectively, while those of seeds from Gansu Chengxian reduced. Conclusion： Treated with PEG, GA3, KNO3-KH2PO4 under proper concentration, seed vigor, seed resistance under drought stress would increase.