锁阳多糖对D-半乳糖衰老小鼠血细胞和脑细胞染色体端粒长度的影响

Effect of Cynomorium songaricum polysaccharide on telomere length in blood and brain of D-galactose-induced senescence mice

目的:研究锁阳多糖(CSP)对D-半乳糖衰老模型小鼠血液及脑组织细胞染色体末端端粒长度的影响,观察锁阳多糖的抗衰老作用机制。方法:昆明小鼠腹腔注射D-半乳糖(500 mg.kg-1.d-1)制作衰老模型,动物分成正常组、模型组、CSP低(20 mg.kg-1)、中(40 mg.kg-1)、高(80 mg.kg-1)剂量、黄芪多糖(40 mg.kg-1)各组,连续灌胃给药56 d后,应用荧光实时定量PCR方法测定各组小鼠血细胞及脑组织端粒的相对长度。结果:高、中剂量CSP组血细胞相对端粒长度T/Sratio分别为1.64±0.36,1.33±0.28,较衰老模型组1.01±0.13明显增加(P<0.01),脑组织高、中、低剂量CSP组分别为3.34±0.58,2.30±0.75,1.55±0.58,较衰老组1.04±0.33均明显增加(P<0.01)。结论:CSP可以通过抑制D-半乳糖衰老小鼠血细胞和脑细胞染色体末端端粒长度的缩短,从而延缓组织细胞的衰老进程。

Objective： To study the effects of Cynomorium songaricum polysaccharide （CSP） on telomere length in blood and brain tissues of aged mice in order to provide some evidence for CSP~ development and applying in the clinical uses. Method： Kunming mice were intraperitoneal injected D-galactose （500 mg· kg^-1· d^- 1 ） to make the aging models, and different dosages of CSP （20, 40, 80 mg· kg^-1 ） were given by garage for 56 days. The average length of telomere was determined by real-time fluorescence quantitative PCR. Result： The relative T/S ratio of the group high and middle dosages of CSP in blood were 1.64 ±0. 36 and 1.33 ±0. 28, respectively, and higher than that of the group of senescence 1.01 ±0. 13 （P 〈0. 01 ）. Values of the group of high, middle, and low dosages of CSP in brain tissues were 3. 34 ±0. 58, 2. 30 ±0. 75 and 1.55 ±0. 58, respectively, and significantly higher than that of the group of senescence 1.04 ±0. 33 （ P 〈 0. 01 ）. Conclusion： CSP can exert the anti-aging effects by increase telomere length of senescence mice.