半边旗二萜化合物5F对HO-8910PM细胞中Nr1d1表达的影响

Effect of 5F from Pteris semipinnata on expression of Nr1d1 in HO-8910PM cell line

目的:研究半边旗二萜化合物5F(5F from Pteris semipinnata L,PsL5F)对人高转移卵巢癌HO-8910PM细胞中核受体超家族成员1d1(nuclear receptor subfamily 1,group D,member 1,Nr1d1)表达的影响,探讨其抗肿瘤的可能作用机制。方法:培养HO-8910PM细胞,用0.1%DMSO,100μmol.L-1PsL5F分别处理HO-8910PM细胞24 h后,提取RNA和总蛋白,应用肿瘤基因芯片和荧光定量实时PCR检测PsL5F对Nr1d1 mRNA表达的影响;Western blot法分析PsL5F对Nr1d1蛋白表达水平的影响。结果:肿瘤基因芯片结果显示,100μmol.L-1PsL5F处理组中Nr1d1 mRNA表达水平是对照组的26.17倍,荧光定量实时PCR结果与芯片结果吻合,PsL5F处理组中Nr1d1 mRNA表达水平是对照组的(35.34±1.07)倍(P<0.01),并且PsL5F处理组中Nr1d1蛋白表达水平是对照组的(7.71±0.43)倍(P<0.01)。结论:PsL5F能明显上调HO-8910PM细胞中Nr1d1的表达,提示其与PsL5F抗肿瘤作用密切相关。

Objective： To investigate the effects of PsL5F （ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid, an extract from Pteris semipinnata L） on the expression of nuclear receptor subfamily 1, group D, member 1 （Nr1d1） in highly metastatic ovarian carcinoma HO-8910PM cells, and its mechanisms. Method： Microarray Chip was used to examine the level of Nr1d1 mRNA expression on HO-8910PM ceils treated with PsL5F. Fluorescent quantitative real-time PCR assay and Western blot were performed to verify the effects of Psl.SF on Nr1d1 mRNA and protein expression. Result： After 24 h treatment of 100 μmol·L^-1 PsL5F, the mRNA and protein levels of Nrl dl in HO-8910PM cells were 35.34 ±1.07 and 7.71 ±0.43 times compared to those of control group（ P 〈 0.01, P 〈 0.01 ）, respectively. Conclusion： PsL5F can up-regulate siguificantly the expression of Nr1d1 in HO-891OPM cells. Antitumor effects and its mechanisms of PsL5F in HO-8910PM cells may be involved in the up-regulation of Nr1d1 expression.