转Bt基因杨树对杨扇舟蛾的抗性

Resistance of Transgenic Bt Poplar to Clostera anachorta

通过室内饲养和组织切片,研究了表达Cry1A杀虫蛋白的中嘉8号杨树转Bt基因植株对杨扇舟蛾的抗虫效果。结果表明:随着饲养时间的延长,幼虫死亡率和体重抑制率呈上升趋势,处理2d仅分别为10%和6.85%,处理4d分别增加到48.33%和27.93%,处理10d分别达81.67%和63.03%。此外,处理12d取食非转基因杨树幼虫化蛹率100%,而取食转Bt基因杨树幼虫的化蛹率仅为18.18%。组织切片观察表明,取食转Bt基因杨树叶片幼虫的中肠上皮细胞排列松驰,与肠壁基底膜分离,细胞界限较模糊,部分上皮细胞形成空泡。因此,初步认为转Bt基因杨树对杨扇舟蛾幼虫的抗性表现为毒杀作用和抑制其生长发育。

Bio-assays and tissue section examinations were conducted for determining the resistance of transgenic poplar （ Populus deltoides, I-63 × I-69） expressing Bt CrylA toxin to Clostera anachorta. The results showed that larvae fed with the transgenic poplar leaves showed increasing mortalities and weight inhibition rates with the extension of exposure period, being 10% and 6.85% at 2 d, 48.33% and 27.93% at 4 d, and 81.67%, 63.03% at 10 d, respectively. Pupation rate of larvae fed with non-Bt and Bt transgenic poplar for 12 d was 100% and 18.18%, respectively. The midgut epithelium cell of the larvae exposed to transgenic popular was loose and partially vacuolar, and the cell boundary was vague. It is suggested that the transgenic Bt poplar shows insecticidal and growth-inhibitory effects on C. anachorta .