哈茨木霉在水稻体内的定殖及其对水稻纹枯病抗性的影响

Colonization of Trichoderma hazianum in Rice Tissue and Its Effects on Rice Resistance against Sheath Blight

以具有抗腈菌唑标记哈茨木霉TUV-13菌株的分生孢子悬浮液对水稻种子分别进行浸种、蘸根、叶面接种,栽培至2叶1芯期,从秧苗各组织分离回收菌株TUV-13。对经菌液处理的秧苗进行显微及超微观察结果表明,浸种处理能使TUV-13菌株在水稻秧苗的根、茎、叶中稳定定殖;而蘸根处理、叶面接种,TUV-13菌株只能在处理的局部区域定殖。TUV-13菌株定殖后,水稻秧苗与抗病相关的酶活性显著提高,对水稻纹枯病防效达82.9%。

The conidial suspension of Trichoderma hazianum isolate TUV-13 labeled with myclobutanil resistance was applied in rice respectively by seed soaking, leaf spraying, and root dipping. Reisolation from different organs at 2 leaves and 1 shoot seedling stage and observations with light microscope and SEM scanning were conducted to check the colonization. Results showed that TUV-13 colonized roots, stems and leaves of rice steadily when seed was soaked but only a few a the colonization occurred when inoculated by root dipping and leaf spraying. Investigation on activities of POD, PPO and PAL enzymes indicated that they were increased significantly after TUV-13 colonization, and rice sheath blight was reduced by 82.9 %.