人参皂甙Re对异丙肾上腺素致家兔室性心律失常的保护作用

Protective effect of ginsenoside Re on isoproterenol-induced triggered ventricular arrhythmia in rabbits

目的探讨人参皂甙Re对异丙肾上腺素致家兔实验性室性心律失常的保护作用。方法异丙肾上腺素诱导产生触发性室性心律失常模型,家兔分为正常对照组、模型组、阳性对照组(异搏定0.4mg/kg)和实验组(人参皂甙Re20,10或5mg/kg),观察转为窦性节律持续时间,实验结束取左心室部分心肌组织行光学显微镜和电子显微镜检查。结果①异博定组维持窦性节律的时间为177.00±5.66s,人参皂甙Re20,10,5mg/kg处理组维持窦性节律的时间分别是177.83±5.31s、21.00±2.83s、4.50±1.64s。②心肌病理切片表明人参皂甙Re对持续室性心律失常的心肌有显著保护作用。结论人参皂甙Re可用于临床触发性室性心律失常的治疗,可拮抗儿茶酚胺类药物对心肌的毒性作用。

Objective To study the anti-arrhythmie efficacy of ginsenoside Re （GSRe） and its protective effects against myocardial injuries in rabbits with isoproterenol-induced triggered ventricular arrhythmia （TVA）. Methods TVA model was prepared by intravenous injections of isoproterenol at a constant speed of 5 mg/kg/min. When TVA appeared, rabbits were randomly injected with GSRe （5, 10 or 20 mg/kg）, verapamil （0. 4 mg/kg） or placebo. The duratiou of maintaining sinus rhythm was observed. Meanwhile, isoproterenol was continued to be injected at a constant speed of 5 mg/kg/min. After 1 hr of isoproterenol injection, the rabbits were sacrificed. Cardiac muscles in the cuspidate position of the left ventricle were sampled for optical microscopy and electron microscopy. Results GSRe and verapamil treatment restored sinus rhythm. The duration of sinus rhythm was 177.00 ± 5.66 s within 3 minutes in the verapamil treatment group and was 177.83 ± 5.31, 21.00 ± 2.83 and 4.50 ± 1.64 s, respectively, in the 20, 10 and 5 mg/kg GSRe treatment groups. Histopathologic examination demonstrated that GSRe treatment （20 and 10 mg/kg） alleviated myocardial injuries induced by TVA. Conclusions GSRe has anti-arrhythmic efficacies and protective effects against myocardial injuries in rabbits with TVA. It may therefore be a possible therapy for TVA.