摘要
目的探讨哮喘大鼠发病过程中碱性成纤维细胞生长因子(b-FGF)和核因子-κB(NF-κB)在气道内表达的变化,及布地奈德的干预对其表达的影响。方法清洁级Sprague-Dawley雄性大鼠45只,随机分为对照组、哮喘组、干预组。观察卵蛋白激发及用布地奈德干预后哮喘大鼠气道的病理变化;免疫组织化学染色观察b-FGF和NF-κB蛋白表达的变化。结果布地奈德干预减轻了哮喘大鼠气道损伤。b-FGF和NF-κB在对照组中呈弱阳性表达,在哮喘组中表达明显增加,使用布地奈德进行干预后b-FGF和NF-κB的表达明显降低(111.61±5.52vs126.21±6.46;110.65±8.71vs134.15±9.42;P<0.05),且二者呈正相关性。结论b-FGF和NF-κB可能与哮喘大鼠气道重塑相关,布地奈德改善哮喘大鼠的气道重塑可能与降低b-FGF和NF-κB的表达有关。
Objective To study the expression of basic fibroblast growth factor （b-FGF） and nuclear factor-κB （NF-κB） in the airway and the effect of budesonide on their expression in rats with asthma. Methods Forty-five Sprague- Dawley male rats were randomly divided into three group： placebo control, untreated asthma, and budesonide-treated asthma. Asthma was induced by intraperitoneal injection of 10% ovalbumin （OVA） on days 1 and 8 and then challenged by inhalation of 1% OVA aerosol. The budesonide-treated asthma group received an inhalation of budesonide （ 1 mg） 30 minutes after OVA challenge. The pathological changes of the airway were assessed, and the expression of b-FGF and NF-κB in the airway was assayed by hematoxylin and eosin staining and immunohistochemistry. Results Budesonide treatment alleviated airway injuries. Compared with the control group, b-FGF and NF-κB expression in the airway in the untreated asthma group increased significantly （ P 〈 0. 05 ）. The budesonide-treated asthma group demonstrated significantly decreased b-FGF （111.61±5.52 vs 126.21 ± 6.46; P 〈 0.05） and NF-κB expression （ 110. 65 ± 8.71 vs 134. 15 ± 9.42 ; P 〈 0. 05 ） in the airway as compared with the untreated asthma group, b-FGF expression was positively correlated to NF-κB expression in the budesonide-treated group. Conclusions b-FGF and NF-κB may be associated with airway remodeling in rats with asthma. Budesonide can improve airway remodeling, possibly by decreasing the expression of b- FGF and NF-κB.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2009年第5期393-396,共4页
Chinese Journal of Contemporary Pediatrics
