与黄瓜M基因连锁的三个共显性标记

Three Co-dominant Markers Linked to M Gene in Cucumis sativus

利用黄瓜近等基因系纯雌株WI1983G(基因型为FFMM)与两性花株WI1983H(基因型为FFMM)为亲本,以WI1983H为回交亲本构建了BC1代分离群体,采用SSR及SCAR分子标记技术,获得了3个与M基因紧密连锁的标记SSR23487、SCAR123和SSR19914,它们与M基因的遗传距离分别为0.28、0.94和3.20cM,两侧标记SSR23487和SCAR123将M基因定位在1.22cM的遗传区间内。这3个分子标记的获得不仅可以用于分子标记辅助选择育种,而且为最终M基因的克隆打下了基础。

A segregating cucumber BC1 population was developed using the nearly isogenic lines WI1983G （gynoecious, FFMM） as donor and WI1983H （hermaphroditic, FFMM） as recurrent parent. With screening of polymorphism of one SCAR and, 2 112 SSR markers, three polymorphic co-dominant markers SSR23487, SCAR123 and SSR19914 were found to be tightly linking to the M/m gene, with a distance of 0. 28, 0. 94 and 3.20 cM, respectively. The interval between SSR23487 and SCAR123, which locate on each side of the M/m gene, was 1.22 cM. These markers not only could be useful in markers-assisted selection for gynoecious plant, but also provide a solid basis for isolation of the sex,determining M/m gene in cucumber.