云锡肺癌和其他人群肺癌N-ras原位分子杂交检测的比较研究

Comparative Study of N-ras Situ Hybridization Detection between Yunnan Tin Lung Cancer and Other Lung Cancer Populations

用地高辛标记Ｎ－ｒａｓｃＤＮＡ原位分子杂交的方法，检测２０例云锡矿工肺癌（Ｍ组）、２０例非云锡地区非矿工肺癌（ＮＭ组）以及１０例死于非肺部疾患者的尸解肺组织（Ｃ组）中Ｎ－ｒａｓ癌基因的过表达情况．结果发现：Ｍ组、ＮＭ组和Ｃ组过表达率分别为５５％（１１／２０），８０％（１６／２０）和１０％（１／１０）．结果提示：Ｎ－ｒａｓ癌基因的过表达与云锡肺癌和非云锡肺癌的发生有关，且尽管云锡矿工肺癌发病因素具有特殊性，但其Ｎ－ｒａｓ基因的过表达与非云锡地区其他人群肺癌相比无特异性．同时。

With a method of N-ras cDNA situ hybridization marked by digoxin, N-ras oncogene expression was detected in 20 samples of miner lung cancer tissue (M group), in 20 samples of non-miner lung cancer tissue (NM group) and in 10 samples normal lung tissue (C group). As a result the expression rates were 55% (11/20), 80% (16/20) and 10% (1/10) respectively in M, NM and C groups. The results demonstrated that N-ras oncogene high expression was related to the carcinogenesis of miner lung cancer and non-miner lung cancer. Although specific in its occurrence factors, N-ras oncogene high expression of miner lung cancer was not special compared with that of non-miner lung cancer. Positive reaction was also found in hyperplastic epithelium of pericancerous lung tissue.