摘要
目的建立B病毒核酸的PCR检测方法。方法设计引物,用PCR方法扩增B病毒,并验证其灵敏性和特异性。结果引物P1、P2及P3、P4在以B病毒为模板时有特定大小的目的片段出现,在以其他病毒为模板时无目的片段出现;引物P5、P6以B病毒和人单纯疱疹病毒I型(HSVI)为模板能扩增出382bp的产物,经SacⅡ酶切后,B病毒产生176bp和206bp的两个片段,HSVI无变化。结论通过PCR方法成功的区分开B病毒,并且鉴定区分了B病毒和HSVI。
Objective To establish PCR assay for detection of B virus DNA. Method We designed primers, amplified B virus by PCR and verified its sensitivity and specificity. Results There are predicted products when P1, P2 or P3, P4 as primer and BV DNA for template, but there are no PCR products when other viruses DNA as templates ; There are 382 bp products when P5, P6 as primers , BV and HSVI DNA as templates. Cut by Sac Ⅱ there are 176 bp and 206 bp products when BV DNA as template. The products cannot be cut off when HSVI DNA as template. Conclusion We discriminate B virus by PCR and distinguish B virus from HSVI.
出处
《实验动物科学》
2009年第2期28-32,共5页
Laboratory Animal Science
关键词
B病毒
PCR
酶切
B virus
Polymerase chain reaction
Cut by restriction enzyme
