地西泮和戊巴比妥钠联合使用对小鼠麻醉的效果观察

The Effect of Combining Diazepam and Sodium Pentobarbital on Anesthesia in Mice

目的通过给小鼠注射地西泮和戊巴比妥钠的方法,探讨复合麻醉对小鼠的麻醉效果。方法将ICR小鼠随机分为对照组和试验组。对照组小鼠皮下注射生理盐水(0.1mL/10g)和试验组小鼠皮下注射地西泮(1mg/kg)后,分别放入小鼠自主活动箱内,记录小鼠的自主活动次数。然后,两组小鼠均腹腔注射戊巴比妥钠(50mg/kg),记录小鼠翻正反射消失时间和翻正反射恢复时间。结果注射地西泮小鼠自主活动次数显著少于生理盐水组P<0.01)。地西泮和戊巴比妥钠联合麻醉小鼠比戊巴比妥钠单独麻醉小鼠的入睡时间显著缩短P<0.01),睡眠时间显著延长(P<0.01)。结论地西泮和戊巴比妥钠联合麻醉明显提高小鼠的麻醉效果。

Objective To study the effect of combinational anesthesia, the mice were injected with diazepam and pentobarbital sodium. Methods ICR mice were randomly divided into control group and the test group. Control group of mice were subcutaneously injected with normal saline （0.1 mL/10 g）, and experimental group of mice were subcutaneously injected with diazepam （1 mg/kg）. Spontaneous activity was examined by an apparatus to record the movement of mice in a box. And then, the two groups of mice were injected with pentobarbital sodium （50 mg/kg）. The disappearing time and recovery time of righting reflex were recorded. Results The spontaneous activity in the mice injected with diazepam was significantly lower than the mice injected with saline （ P 〈 0.01 ）. For the mice injected both diazepam and sodium pentobarbital, the time to fall asleep was significantly shorter （P 〈 0.01 ） and the sleep time was significantly longer （ P 〈 0.01 ） than the mice injected sodium pentobarbital only. Conclusion Combination of diazepam and sodium pentobarbital can significantly improved the anesthesia effect in mice.