高效液相色谱法测定对乙酰氨基酚片的含量

Content determination of Paracetamol Tablets by HPLC

目的:建立对乙酰氨基酚片含量的高效液相色谱测定法。方法:色谱柱为依利特C18柱(4.6mm×250mm,5μm),流动相为磷酸盐缓冲液(pH4.5)-甲醇(80:20),流速为1.0ml/min,检测波长为254nm,柱温为30℃。结果:对乙酰氨基酚在0.05～0.30mg/ml浓度范围内呈良好线性关系(r=0.9999),平均加样回收率为100.5%(n=5),RSD为1.2%。结论:该方法准确、可靠,与标准法比较,结果较为满意,可用于对乙酰氨基酚片的含量测定。

Objective： To establish a HPLC method for determination of Paracetamol Tablets. Methods： Elite C18 column （4.6 mm×250 mm, 5 μm） was used with a mobile phase composed of phosphate buffering solution（pH4.5）-methanol（80： 20） at the flow rate of 1.0 ml/min, the detection wavelength was 254 nm and the temperature of the column was 30℃. Resuits： The calibration curve of paracetamol was linear in the range of 0.05-0.30 mg/ml,r=0.999 9, the average recovery rate was 100.5% with a RSD of 1.2%（n=5）. Conclusion： This method is accurate, realiable and can be used for the determination of Paracetamol Tablets.