摘要
目的探讨外源性瘦素对重症急性胰腺炎大鼠肺组织核因子-κB、诱生型一氧化氮合酶、NO表达的影响,以及瘦素在SAP肺损伤的治疗效果及作用机制。方法36只大鼠随机分为正常对照组、SAP组和外源性瘦素治疗组。以5%牛磺胆酸钠制作SAP模型。造模后6h取材,检测肺组织NF-κB活性、NO、iNOS含量,同时观察血浆淀粉酶及其病理改变等。结果正常对照组几乎无NF-κB活化,SAP时NF-κB活化及iNOS、NO高表达。瘦素对SAP肺组织NF-κB活性有抑制作用,可使iNOS活性表达降低,并可降低血浆淀粉酶。肺病理可见:给予外源性瘦素后,SAP大鼠肺组织损伤明显减轻。肺组织NF-κB活化与iNOS表达及肺组织病理评分均呈正相关。结论肺组织NF-κB活化及iNOS过度表达可能是SAP肺损害发生的原因之一。瘦素可能通过抑制NF-κB活性途径减少iNOS的表达,从而减少过量NO的生成,减轻SAP肺组织损害。
Objective To explore the relationship between expression of iNOS,NO,NF-κB activation of lungs tissue and lungs injury in rats with SAP and the effect of leptin on SAP. Methods 36 rats were randomized into the three group：normal group,SAP group,leptin treatment group. The SAP model was induced by injection of 5% sodium taurocholate solution intobiliopancreatic duct. All the animals were sacrificed in 6h. Activation of NF-κB was determined and expression of NO was assayed. Mean-while,iNOS,plasma level of amylase,and histological grading were determined. Results In SAP group,the NF-κB activity,the NO levels and the iNOS expression increased markedly.In Leptin group, the activation of NF-κB was prevented and plasma level of amylase, and content of NO and iNOS remarkably decreased. Conclusion Early blockage of NF-κB activation with leptin is effective for reducing severity of the lungs injury.Leptin could reduce iNOS expression and NO level by inhibiting the NF-κB activation. Leptin may be beneficial marker for SAP.
出处
《中国现代医生》
2009年第15期68-69,71,共3页
China Modern Doctor
