社区环境中食源性沙门菌的分布菌型及耐药性消除试验

The Distribution Types of Foodborne Pathogenic Saimonella in Community Environments and the Experiments of Drug Resistance Elimination

目的研究H市社区食源性沙门菌的分布、菌型、耐药性及耐药性消除。方法按GB/T4789-2003分离、鉴定沙门菌并用标准血清分型,纸片琼脂扩散(K-B)法测定耐药性,SDS高温处理消除耐药性。结果自5类食品(130份样本)分离到沙门菌47株,属3群6个血清型,主要是鼠伤寒沙门菌(25株,53.19%),其次是肠炎沙门菌、猪霍乱沙门菌。三型沙门菌的多重耐药株分别是60.00%(15/25)、33.33%(3/9)、28.57%(2/7)。经SDS高温处理可使80.00%的耐药株的耐药性逆转。结论H市食源沙门菌菌型集中,主要是鼠伤寒沙门菌,耐药性严重,它们中有60.00%菌株是多重耐药株,但对哌拉西林、亚胺培南、环丙沙星敏感。SDS高温处理可使80.00%菌株的多重耐药性消除。

Objective To studying the distribution, types, drug resistance and drug resistance elimination of foodborne pathogenic Saimonella in community environments of the H city. Methods According to GB/T4789-2003 to separating and identifying foodbome pathogenic Saimonella, and serotyping with standard serum. Applying scrip diffusion method（K-B ） to determine the drug resistance of Salmonella. Using SDS megatemperature handling to eliminate the drug resistance. Results 47 strains of Salmonella had been separated from five kinds of foods（ 130 samples ）, belong to 3 groups and 6 serotypes. Major strains were Salmonella typhimuria（25 strains, 53.19% ）, next strains were Salmonella en- teritidis and Salmonella eholeraesuis. The percentage of multidrug resistant strains in these three types of Salmonella were 60.00%（ 15/25 ）, 33.33%（3/9 ）, 28.5%（2/7）. The drug resistance of eighty percent persisters could be reversed by SDS megatemperature handling. Conclusion The types of foodborne pathogenic Saimonella in the H city were centralized. Salmonella typhimuria were major strains,the drug resistance of which was severe, sixty percent strains of which were multidrug resistant, but these strains were sensitive with regard to piperacillin, imipenem, ciprofloxacin. The multidrug resistance of eighty percent persisters could be eliminated by SDS megatemperature handling.