摘要
目的建立测定蛇床子洗液中蛇床子素的方法。方法使用高效液相色谱法，色谱柱为DiamOnsilC18柱（250mm×4．6mm，5μm），检测波长322nm，流动相为甲醇-水（78：22）。结果在12．08μg-96．64μg范围内有良好的线性，平均回收率为90．11％，1LSD为1．98％（11=5）。结论该法操作简便，结果；隹确，灵敏度高，重现性好．可用于该洗液的质量控制。
Objective to establish the determination method for osthole in FURUCTUS CNIDII Lotion. Methods HPLC was used with Diamonsil C18column （250mm×4.6mm, 5μm）, detection wavelength at 322 nm, mobile phase： methanol-water （78： 22）, flow rate： lml/min. Results Osthole has good linear relationship between 12.08 μ g -96.64 μ g , the average recovery was 90.11% and RSD was 1.98% （n=5）. Conclusion The method is simple, sensitive and reliable to carry out to control the quality of this Lotion.
