甜瓜果实醇酰基转移酶基因的克隆及表达分析

Cloning and Expression Analysis of Alcohol Acyl-transferase Gene from Muskmelon Fruit

根据在GenBank中登录的烟草、番茄等的醇酰基转移酶基因的保守序列设计引物,利用RT-PCR和RACE(Rapid Amplification of cDNA Ends,cDNA末端快速扩增)技术从甜瓜自交系M01-3花后25 d的果实总RNA中扩增到甜瓜醇酰基转移酶基因全长cDNA。该基因全长为1 429 bp,开放读码框为1 383 bp,编码461个氨基酸,GenBank中登录号为EU431334。利用荧光定量PCR方法进行了该基因在果实发育过程中的表达特性分析,结果表明该基因在花后15 d果实中的表达量最低,成熟果实中表达量最高。

PCR primers were designed based on the conserved domain of some alcohol acyl - transferase genes in C, enBank. The full - length eDNA clone was amplified from the total RNA isolated from 25 - day muskmelon fruit after pollination using RT - PCR and RACE. The clone contained 1 429 nucleotides with an open reading frame of I 383 nucleofides. Quantitative real - time RT - PCR analysis indicated that CmAATI mRNA accumulation showed the minimum level on the 15th day after pollination and reached the highest level in mature fruit.