抗戾饮对IBV感染模型组织中N基因相对表达量的影响

Effects of Kangliyin on N Gene Relative Expression in the Infectious Bronchitis Virus Infected Model

目的利用实时荧光定量PCR方法研究具有清热利湿和胃化痰之功的抗戾饮对呼吸道冠状病毒——传染性支气管炎病毒(IBV)感染雏鸡模型组织中病毒N基因相对表达量的影响。方法将7日龄雏鸡按体重分层,随机分为6组,即空白对照组、模型组、抗戾饮高、中、低剂量组和更昔洛韦组。利用滴鼻、点眼方式接种IBV,从接种后第1天开始,模型组和空白对照组分别灌胃给予无菌生理盐水,抗戾饮高、中、低剂量组分别给予抗戾饮水煎剂10,20,40g·kg-1·d-1,更昔洛韦组给予更昔洛韦0.004g·kg-1·d-1,共给药5d。观察IBV感染模型的临床症状,取肺与气管组织用实时荧光定量PCR方法检测各组模型组织中IBV的N基因相对表达量的变化。结果与模型组相比,抗戾饮能有效改善IBV模型的一般症状及肺与支气管组织的病理损伤,并能显著降低模型组织中IBVN基因的相对表达量。结论抗戾饮可以有效地抑制模型组织病毒的复制,修复组织的病理损伤,改善IBV感染模型的症状。

Objective To obserce the therapeutic effect of Kangliyin （KLY, the compound Chinese herbal formulation with the functions of clearing heat and damp elimination, regulating stomach and dissipate phlegm） on the models with infection of infectious bronchitis virus （IBV）, and to evaluate its effect on the IBV N gene relative expression by using real- time florescent quantitative polymerase chain reaction （PCR）. Methods Seven- day -old chicks were inoculated with IBV by the nasal and ocular administration. The chicks were divided into 6 groups by stratified random sampling according to the body weight. The 6 groups were blank control group, model group, three KLY treatment groups（ receiving KLY at the dosage of 10 g/kg·d^-1, 20 g/kg·d^-1 or 40 g/kg·d^-1, respectively）, and positive control group （ganciclovir at the dosage of 0. 004 g/kg·d^-1）. The chicks in the model group and blank control group received normal saline, and the medication groups received the corresponding medicine according to the experimental design from the next day of inoculation, and the treatment lasted 5 days. The relative quantity of IBV N gene expression in the lung and bronchial tissues were assayed by real -time florescent quantitative PCR, and the clinical manifestations and the pathological changes of all groups were also examined. Results Compared with the model group, KLY could significantly inhibit the IBV N gene expression, improve the general clinical symptoms and respiratory symptoms, and improve the pathological changes in the bronchus and lung of the IBV infected model. Conclusion KLY could effectively inhibit the viral replication, repair the pathological damage in the bronchus and lung, and improve the general clinical symptoms in the IBV infected model, which is beneficial to the recovery of the chicks from IBV infection.