马钱子总生物碱脂质体的含量与包封率测定

Determination of Content and Encapsulation Efficiency of Liposomes Containing Total Alkaloids from Seed of Strychnos nux-vomica L.

目的建立测定马钱子总生物碱脂质体含量和包封率的方法,用于处方与工艺评价。方法采用氨性氯仿法提取马钱子总生物碱并分析其成分,采用硫酸铵梯度法制备马钱子总生物碱脂质体。以士的宁为对照品,采用紫外分光光度法,在254nm测定用破膜剂溶解的马钱子总生物碱脂质体的吸光度确定其含量。采用葡聚糖凝胶柱分离脂质体和游离药物,分别测定含量后计算包封率。结果马钱子总生物碱提取物平均得率为3.49%,在总生物碱提取物中士的宁和马钱子碱分别占46.76%和26.51%。士的宁标准曲线的范围是2.4～20μg/mL,加样回收率在96.66～102.62%范围内。葡聚糖凝胶柱层析的回收率为100.85±0.79%(n=3)。结论所建立的方法简便快捷稳定,可以满足马钱子总生物碱脂质体质量控制的需要。

Objective To establish the method to determine the content and encapsulation efficiency of liposomes containing total alkaloids from seed of Strychnos nux - vomica L. Methods The total alkaloids were extracted from seeds of Strychnou nux - vomica L. and the content was analyzed. Liposomes containing the total alkaloids were prepared by ammonium sulfate transmembrane gradients. The total alkaloid content of dissolved liposomes was determined by UV spectrophotometry at 254 nm, which was performed with strychnine as the reference substances. The encapsulated and free drugs were separated with Sephadex G - 50 column, and then their contents were determined to calculate the encapsulation efficiency. Results The average extracted efficiency of total alkaloids from seed of Strychnos nux- vomica L. was 3.49 %. Strychnine and brucine accounted for 46.76 % and 26.51% in the extract, respectively. Strychnine at the concentration of 2.4 - 20 μg/mL had a good linear relationship with the absorbance. The recovery of strychnine was in the range from 96. 66 % to 102.62 %. The recovery of Sephadex G - 50 column was （ 100. 85 ±0.79） % （ n = 3）. Conclusion The established method is simple, accurate and stable, and can be used for the quality control of liposomes containing total alkaloids from seed of Strychnos nux - vomica L.