摘要
目的测定裸花紫珠中木犀草素的含量。方法采用HPLC法,色谱条件为Alltima C18柱(250mm×4.6mm,5μm),甲醇-水-磷酸(55∶45∶0.4)为流动相,流速1mL/min;检测波长348nm,柱温:室温。结果木犀草素在0.03246~0.1298μg范围内线性关系良好(r=0.9995);平均回收率为99.52%,RSD=1.58%。结论该方法简便可行,重复性好,可用于裸花紫珠的质量控制。
Objective To determine the content of luteolin in Callicarpa nudiflora Hook. et Am.. Methods HPLC was performed with chromatographic conditions as follows: Alhima Cla column(250 mm × 4.6 mm, 5 μm)with column temperature at 25 ℃ , the mobile phase of methanol -water -phosphate(55: 45: 0. 4), flow rate at 1 mL/min, and the detection wavelength at 348 nm. Results The linearity of luteolin was good in the range of 0. 032 46 - 0. 129 84 μg( r = 0. 999 5). The average recovery was 99.52 % and RSD was 1.58 %. Conclusion The method is simple, feasible and reproducible, and can be used to control the quality of Callicarpa nudiflora Hook. et Arn..
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第3期271-272,共2页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
江西省自然科学青年基金项目(2008GQY0099)
关键词
裸花紫珠
木犀草素
HPLC法
Callicarpa nudiflora Hook. et Arn
Luteolin
HPLC