PGC-1α基因Gly482Ser多态性与2型糖尿病发病的相关性研究

Association of Peroxisome proliferator activated receptor-γ coactivator-1α(PGC-1α) Gly482Ser polymorphism with type 2 diabetes mellitus in Chinese women

[目的]研究过氧化物酶体增殖物激活受体γ共活化物-1α(PGC-1α)Gly482Ser基因多态性与2型糖尿病发病的相关性。[方法]选取大连居住的汉族人2型糖尿病病人(DM)140例和非糖尿病对照(NC)88人;用聚合酶链反应(PCR)-限制性内切酶技术检测PGC-1αGly482Ser基因型。[结果]DM组Ser/Ser基因型分与NC组比较差异有显著性意义(χ2=10.969,P=0.004),按性别分层后男性DM组与NC组比较差异无显著性意义(χ2=3.601,P=0.165),而女性DM组与NC组比较差异有显著性意义(χ2=11.02,P=0.004)。在NC组和DM组,随Ser等危基因增加,空腹C肽和2 h C肽呈逐渐降低趋势,但只有Ser/Ser与Gly/Gly两基因型之间相比差异有显著性意义(P<0.05)。在NC组,各基因型之间的胰岛素抵抗之间比较差异无没有显著性意义(P>0.05)。[结论]PGC-1 Gly482Ser多态性可能与女性2型糖尿病的发病相关,Ser/Ser型携带者C肽水平更低。

[ Objective] To study if PGC - 1α Gly482Ser polymorphism is related to type 2 diabetes mellitus in Chinese. [ Methods ] One hundred and forty type 2 diabetes （DM） and 98 non - diabetic controls （NC） were selected. Genotyping of PGC -1α Gly482Ser was determined with PCR amplification, followed by MspI digestion. [ Results] PGC - 1α Ser/Ser genotype frequency in DM was significantly higher than that in NC （29% vs 12.5% , χ^2 = 10. 969, P =0. 004）. Upon stratification with sex Ser/Ser genotype was significantly higher in DM women than NC women （χ^2 = 11.02, P = 0. 004 ）, while it was not so in men. Both in NC and DM, fasting C - peptide and 2 hour postprandial C - peptide levels were lower in subjects with Ser/Ser genotype than in subjects with Gly/Gly genotype （ P 〈 0.05 ）. There was no significance for insulin resistance among different genotypes both in DM and NC groups （P 〈 O. 05 ）. [ Conclusion ] The PGC - 1α Gly482Ser polymorphism might be related to the development of type 2 diabetes in Chinese women. Ser/Ser seems to be associated with lower endogenous insulin secretion.