MTA1基因在肝癌细胞系HepG2及其异质性亚系中表达的差异性

Variability of MTA1 expression in hepatocellular carcinoma cell line HepG_2 and its heterogeneous sublines

目的:探讨MTA1基因在肝癌细胞系HepG2及其异质性亚系中的表达水平。方法:采用半定量RT-PCR、荧光定量RT-PCR的方法检测3种细胞中MTA1基因的表达差异;Western blotting检测蛋白水平的表达差异;用细胞免疫组织化学方法观察MTA1蛋白的细胞定位。结果:HepG2-H和HepG2细胞株的mRNA和蛋白表达水平均高于HepG2-L细胞株;细胞免疫组化显示3株细胞的MTA1蛋白均定位于细胞核和细胞浆,以细胞核为主。结论:MTA1基因在具有不同转移潜能的肝癌细胞亚系HepG2-H、HepG2-L中的表达具有明显差异。

Aim： To investigate the expression level of metastasis associated gene-1 （MTA1） in hepatocellular carcinoma cell line HepG2 and its heterogeneous sublines. Methods： The expression level of MTAI in vitro was examined by using semi-quantitative RT-PCR, real-time RT-PCR. The variability of protein expression was measured by Western blotting immunohistoehemistry. Results： The MTA1 levels of mRNA The location of MTA1 in cells was studied by and protein expressing in HepG2-H, HepG2 were higher than those in HepG2-L. MTA1 can be found in the nucleus and cytoplasm of the ceils, predominantly in the nucleus. Conclusion：Expression of MTA1 gene in two heterogeneous sublines that derived from HepG2 with different metastatic potency shows significant differences.