维生素D受体蛋白及其mRNA在人输卵管黏膜上皮的表达

Expressions of the vitamin D receptor protein and its mRNA in mucosa epithelium of human fallopian tubes

目的:探讨人输卵管黏膜上皮维生素D受体(VDR)蛋白及其mRNA的表达。方法:30例输卵管标本取自具有正常妊娠生育史,因子宫肌瘤、子宫腺肌病等行腹式子宫切除术,一并切除输卵管的妇女。输卵管取材包括峡部、壶腹部和伞部,按子宫内膜组织学分期分为增生早期组6例、增生中晚期组9例、分泌早期组7例、分泌中晚期组8例。应用免疫组织化学法和逆转录聚合酶链反应法检测人输卵管黏膜上皮VDR的表达。结果:人输卵管黏膜上皮中检测到VDR蛋白及其mRNA表达。VDR蛋白主要表达于输卵管上皮细胞的细胞核中,部分间质平滑肌细胞核内亦有表达。相同时期各不同部位输卵管上皮细胞VDR蛋白表达无明显差别(P>0.05),随月经周期变化,各不同部位输卵管上皮细胞VDR蛋白表达发生改变,在增生早期和分泌中晚期较低,与之比较,在增生中晚期和分泌早期其表达明显增高(P<0.01),至分泌早期达最高值;壶腹部输卵管黏膜上皮组织VDR mRNA表达在增生早期和分泌中晚期较低,在增生中晚期和分泌早期明显增高(P<0.01)。结论:人输卵管黏膜上皮组织存在VDR蛋白及其mRNA表达,在增生中晚期和分泌早期其表达明显增高,且具有周期性变化。

Aim：To investigate the expressions of vitamin D receptor （VDR） protein and its mRNA in mucosa epithelium of human fallopian tubes. Methods： Samples of fallopian tubes were obtained from 30 women undergoing abdominal hysterectomy with adnexectomy for benign disease such as uterine leiomyoma, adenomyosis in the pelvic cavity. The isthmic, ampullary and fimbrial portions of fallopian tubes were respectively collected. These specimens were divided into 4 groups based on their endometrium histological staging： early-proliferative stage （ n = 6）, mid- and late-proliferative stage （ n = 9）, early-scoretory stage （n = 7）, mid- and late-secretory stage （n = 8 ）. The expression of VDR in mueosa epithelium of human fallopian tubes was determined by immunohistochemistry and reverse transcription polymerase chain reaction methods. Results： VDR protein and expression of its mRNA were detected in mucosa epithelium of human fallopian tubes. VDR positive staining was mainly located in the nucleus of epithelial cells, observed in smooth muscle cells occasionally. There was no significant difference in VDR positive expression among the isthmic, ampullary and fimbrial portions during the same phase of menstrual cycle （P 〉 0. 05）. However, VDR positive expression in the different portions changed with the variation of menstrual cycle. It was in low level during both the early-proliferative and mid- and late-secretory stages, then increased in both the mid- and late-proliferative and early-secretory stages significantly （ P 〈 0. 01 ） and achieved the maximum during the early-secretory stage. The expression of VDR mRNA in ampullary portion of fallopian tubal epithelium was in low level during both the early-proliferative and mid- and latesecretory stages, then obviously increased in both the mid- and late-proliferative and early-secretory stages （P 〈0. 01 ）. Conclusion：The expressions of VDR protein and its mRNA would exist in mucosa epithelium of human fallopian tubes. And the expressions would exhibit a cyclic change. During both the mid- and late-proliferative and early-secretory stages, the expressions of VDR protein and mRNA would increase significantly.