小鼠肺腺癌动物模型的建立

Establishment of a mouse model of pulmonary adenocarcinoma

目的通过建立小鼠肺腺癌动物模型,为下一步筛选肺腺癌形成过程中重要miRNAs提供实验平台。方法采用Lox-stop-lox K-ras G12D小鼠鼻腔吸入AdCre重组腺病毒的方法建立小鼠肺腺癌模型,以分散酶和Ⅳ型胶原酶酶解消化模型小鼠肺组织为单细胞悬液并以免疫荧光染色的方法检测其CD34、Sca-1的表达情况。结果通过Lox-stop-lox K-ras G12D小鼠鼻腔滴入AdCre成功建立了小鼠肺腺癌模型,模型小鼠中有CD34、Sca-1的表达。结论成功建立小鼠肺腺癌动物模型,为后续筛选小鼠肺腺癌发展过程中重要miRNA提供了实验基础。

Objective To establish a model of pulmonary adenocarcinoma in mice,and to supply an experiment platform for screen of important miRNAs in the process of pulmonary adenocarcinoma. Methods The pulmonary adenocarcinoma model was established by intranasal administration of adenovirus Cre to Lox-stop-lox K-ras G12D mice. The lung tissues of model mice were digested with dispase and collagenase IV in combination, and then the expression of Sca-1 and CD34 was detected by the dual-color immunofluorescence. Results The model of pulmonary adenocarcinoma was established successfully, and Sca-1 and CD34 were expressed in the lung of model mice. Conclusion The model of pulmonary adenocarcinoma can be established successfully for further study on screen of important miRNAs in the process of pulmonary adenocarcinoma.