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利用RAP-PCR检测高盐刺激下Shewanella piezotolerans WP3的基因差异表达

Differential Expression of Shewanella piezotolerans WP3 upon High Salinity Stimulation Using RAP-PCR
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摘要 选取高盐刺激为条件,利用实验室模拟极端环境培养条件下的菌体提取RNA,采用差异显示方法RAP-PCR扫描盐刺激下基因的差异表达。结果表明,在高盐刺激下,WP3下调表达的基因是SS1:hypothetical protein;SS2:ferroxidase。上调的基因是SS3:gamme-butyrobetaine antiporter;SS4:hypothetical protein。 The effect of the high salinity shock on the gene expression was performed in Shewanella piezotolerans WP3.The RNA bearing high salinity shock was isolated and then scanned by differential display RAP-PCR.The results showed that the genes encoding hypothetical protein and ferroxidase were induced and the genes anotated as gamme-butyrobetaine antiporter and hypothetical protein were repressed upon high salinity concentration stimulation.
作者 李升康 李金媛 王玉桥
机构地区 汕头大学理学院生物系 国家海洋局生物遗传资源重点实验室
出处 《长江大学学报（自科版）（中旬）》 CAS 2009年第1期64-67,84,共5页 Journal of Yangtze University(Nature Science Edition)
基金 国家大洋开发协会项目(DY7000M-02)
关键词 SHEWANELLA piezotolerans WP3 差异显示 高盐刺激 基因表达 Shewanella piezotolerans WP3 differential display high salinity concentration stimulation gene expression
分类号 Q786 [生物学—分子生物学]
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长江大学学报（自科版）（中旬）
2009年 第1期

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