不同频率电针对急性应激模型C57BL／6小鼠抑郁行为的影响

Effect of differential electroacupuncture frequencies on behavior of acute stress depression model of C57BL/6 mice

目的探讨不同频率电针干预对C57BL／6小鼠急性应激的影响。方法30只C57BL／6雄性小鼠，随机分为模型组、电针2Hz组和电针100Hz组，每组各10只。对不同频率电针干预组，选取“百会”和“印堂”穴，采用频率为2Hz或100Hz、电流强度1mA的方波干预7d，进而对所有小鼠进行强迫游泳实验和悬尾实验，比较各组小鼠强迫游泳和悬尾实验5min内的累积不动时间。结果电针2Hz组小鼠的强迫游泳不动时间[（32．00±14．73）s]和悬尾不动时间[（72．50±20．44）s]均较100Hz小鼠强迫游泳不动时间[（145．60±34．62）s]和悬尾不动时间[（124．22±21．67）s]短，2组之间差异有显著性（P〈0．05）；而且2Hz组小鼠的强迫游泳不动时间和悬尾不动时间均短于模型组强迫游泳不动时间[（129．50±33．94）s]和悬尾不动时间[（142．23±20．05）s]，组间差异有显著性（P〈0．05），但100Hz组与模型组比较，差异无显著性（P〉0．05）。结论频率2Hz的电针对C57BL／6小鼠急性应激抑郁状态有较好预防作用。

Objective To explore the effect of different frequencies interference of electoracupuncture on acute stress of C57BL/6 mice. Methods Thirty male C57BL/6 mice were randomly divided into model group, 2Hz electroacupunture group and 100 Hz electroacupunture group. After interfering 7 days by electroacupuncture, all the mice were carried forced swimming tests and tail suspension test, and the immobility time in 5 minutes were taken down. Results The immobility time of forced swimming test and tail suspension test of 2 Hz eletroacupuncture group（32.00 ± 14.73 ） s, （72.50 ±20.44） s was shorter than those of 100Hz electoracupuncture group （ 145.60 ±34.62） s, （ 124.22 ±21.67 ） s（P 〈 0.05 ） ; and the immobility time of 2 Hz eletroacupuneture group was shorter than that of model group（ 129.50 ±33.94 ） s, （ 142.23 ±20.05 ） s （P 〈 0.05 ） ; but the immobility time of 100 Hz eleetroacupuncture group was no difference with that of model group（P 〉 0. 05 ）. Conclusions The effect of 2 Hz eletroaeupuncture could improve the behaviors of the acute stress of C57BL/6 mice and had the more effectiveness on anti-depression.