利用基因芯片技术对小鼠隐睾相关基因谱研究

Investigation of differential gene expression profiles in mice cryptorchid testes by cDNA microarray

目的:利用基因芯片研究小鼠隐睾相关基因谱的改变.方法:建立小鼠隐睾模型,分别提取正常及异常睾丸组织的mRNA,制备杂交用cDNA探针,在包含小鼠4000条cDNA片段的基因芯片上点样、杂交、洗涤后,通过计算机观察二者表达谱的差异情况,并对其中表达下降的COX8a进行RNA斑点印迹杂交以验证基因芯片结果.结果:通过基因芯片筛选,获得34条与小鼠隐睾相关的基因.RNA斑点印迹支持基因芯片杂交结果.结论:利用基因芯片研究小鼠隐睾睾丸组织与正常睾丸组织差异表达的基因谱可以用于高通量筛选隐睾相关基因以及进一步的研究;COX8a可能在隐睾症的发生与进展过程中起一定的作用.

AIM：To study the differential gene expression pro- files between the normal and cryptorchid mice testes by cDNA microarray. METHODS： BLAC mice were unilaterally produced cryptorchidism artificially. Four weeks later, we extracted mRNA from both normal and cryptorchid testes, produced cDNA probes and employed them on cDNA microarray to detect the differential gene expression profiles. RNA dot blotting was taken on COX8a, a down-expressed gene in the previous hybridization, to validate the microarrays. RESULTS： Thirty-four differential expressed genes related to cryptorchism were found. CONCLUSION： Screening of the differential gene expression profiles between the normal and cryptorehid mice testes by cDNA microarray can be used in the study of cryptorchid-related genes and further researches. COX8a may play a role in the development and progression of cryptorchidism.