硫辛酸对老年大鼠肾缺血再灌注后肺损伤时黏附分子ICAM-1表达的影响

Effect of lipoic acid on expression of ICAM-1 of lung tissue following renal ischemia reperfusion in aged rats

目的:探讨硫辛酸对老年大鼠肾缺血再灌注致肺损伤时黏附分子ICAM-1(Intercellular adhesion molecule-1)表达的影响.方法:复制大鼠肾缺血再灌注损伤(renal ischemia reperfusion injury,RIRI)模型,采用流式细胞技术、逆转录聚合酶链反应(RT-PCR)和Western Blot观察RIRI发生过程中,血液中CD18阳性的多形核白细胞(polymorphonuclear leukocyte,PMN)百分率、肺系数(pulmonary index,LI)与肺通透指数(pulmonary permeation index,LPI)、肺组织形态学的改变、肺组织ICAM-1在mRNA和蛋白水平的变化及硫辛酸对上述指标的影响.结果:大鼠肾缺血再灌注后LI,LPI及CD18阳性细胞数均明显增加,肺组织髓过氧化物酶(myeloperoxidase,MPO)活性增加,ICAM-1表达明显升高,而提前给予硫辛酸可以明显抑制这些变化,从而对肺起一定保护作用.结论:肺组织细胞ICAM-1表达上调及血液中CD18阳性细胞数的增加可能参与RIRI时肺损伤的发生;硫辛酸可减少血液中CD18阳性细胞数,并且下调肺组织ICAM-1的表达,从而减轻肺损伤.

AIM： To investigate the influence of lipoic acid on the expression of ICAM-1 of lung tissue following renal ischemia reperfusion in rats. METHODS： The model of renal ischemia reperfusion injury was duplicated. The percentage of positive CD18 polymorphonuclear leucocytes （PMN） in blood, pulmonary index （LI）, pulmonary permeation index （LPI）, morphological change of lung, changes of mRNA and protein levels were observed by Flow cytometer, RT-PCR and Western blotting. RESULTS： After renal ischemia reperfusion, the number of positive CD18 PMN, LI and LPI obviously increased and the activity of myeloperoxidase in the lungs increased. The expression of ICAM-1 increased obviously. Administration of lipoic acid in advance inhibited these changes and protected the lungs. CONCLUSION： The up-regulation of ICAM-1 expression and the increasing number of positive CD18 PMN in blood probably participate in the lung injury after renal ischemia reperfusion. Lipoic acid may reduce the injury of the lungs by decreasing the number of positive CD18 PMN and down-regulating ICAM-1 expression.