人宫颈癌基因蛋白(HCCR)在人结肠癌中的表达

Expression of human cervical cancer oncogene (HCCR) in colon cancer

目的:检测人宫颈癌基因(HCCR)蛋白在人结肠癌组织中的表达,了解其表达与结肠癌临床病理特征之间的关系.方法:应用免疫组织化学SP法检测20例正常结肠组织、21例腺瘤组织和56例结肠癌组织中HCCR的表达.结果:HCCR在正常结肠组织无表达;腺瘤组织和结肠癌组织中表达率分别为48%和77%,HCCR在结肠腺瘤组织和结肠癌组织中的表达明显高于正常结肠组织,差异有显著性(χ2=13.061,P<0.05),结肠腺瘤组织和结肠癌组织中表达有显著差异(χ2=6.056,P<0.05).HCCR表达与结肠癌组织学类型有关(χ2=5.714,P<0.05),与患者年龄、性别、肿瘤大小、肿瘤部位、组织分化程度、有无淋巴结转移和Dukes分期均无明显相关.结论:结肠腺瘤组织和癌组织中HCCR处于过度表达状态,与结肠癌的早期发生密切相关,HCCR在诊断结肠癌中的价值可能优于CEA和CA199,但不如两者联合检测.

AIM：To detect the expression of human cervical cancer oneogene （HCCR） in human colon cancer, to explore the relations between expression of HCCR with colon cancer and to investigate the role of HCCR in colon cancer. METHODS：The expression of HCCR was detected in 20 patients with normal colonical tissues, 21 patients of colon adenoma tissues and 56 patients of colon carcinoma tissues by immunohistochemiseal SP staining method. RESULTS： No expression of HCCR was observed in normal colon tissue. The positive rate of HCCR expression was 48% in colon adenoma and 77% in carcinoma tissue. The expression of HCCR in colon adenoma tissues and in colon carcinoma tissues was significantly higher than that in normal colon tissues （X^2 = 13. 061 ,P 〈0. 05）, with significant difference between colon adenoma and carcinoma tissue （X^2 = 6. 056 ,P 〈 0.05 ）. The HCCR expression in carcinoma tissue was significantly correlated with the histological type （ X^2 = 5. 714, P 〈 0.05 ）, but not significantly with age, gender, site or size of tumor, histological grade, local lymph node involvement and Dukes stage （ P 〉 0.05 ）. CONCLUSION ： HCCR may play an important role in the early occurrence of colon cancer. Though not as good as joint detection of CEA and CA19-9, HCCR is better than single detection of CEA or CA19-9 in the diagnosis of colon cancer.