摘要
目的观察当归芍药散(DSS)对快速老化小鼠空间学习记忆能力的影响,为当归芍药散治疗阿尔采默病提供实验依据。方法选用雌性SAM—P8鼠36只,分成4组:预防组(3月龄)、预防对照组(3月龄)、治疗组(6月龄)、治疗对照组(6月龄),每组8只。治疗组以DSS(浓度为0.5g/ml)灌胃1个月,预防组自由饮用DSS(0.02%,V/V)4个月,对照组给予冷开水,给药结束后行Morris水迷宫测试。结果Morris水迷宫测试预防组、预防对照组、治疗组、治疗对照组的平均逃避潜伏期分别是(41.29±20.33)s、(62.17±20.36)s、(39.67±19.60)s、(63.00±22.16)s;穿越平台次数分别是(6.13±2.21)次、(2.04±1.42)次、(5.41±2.13)次、(3.12±1.20)次;平台象限游泳距离百分比分别是(51.14±12.38)%、(30.75±14.03)%、(47.11±9.29)%、(33.17±10.45)%。上述指标在预防组、治疗组与其相应对照组比较差异有显著性(P〈0.05)。结论DSS能明显改善老年性痴呆模型SAM—P8小鼠的空间学习记忆能力。
Objective To observe the effect of Danggui Shaoyao San on the learning and memory abilities of SAMP-8 mice, as well as provide scientific basis for its treatment of Alzheimer' s disease. Methods Female SAM-P8 mice were divided into treatment group(6 months, n = 8 ) , treatment control group(6 months, n= 8) ,prevention group(3 months, n=-8) , prevention control group(3 months) randomly. The mice in treatment group were administrated 0. 5ml DSS through stomach for 1 month. The mice in prevention group drank DSS in water(0. 02% , V/V) for 3 months, then were tested learning and memory abilities by Morris water maze. Results Compared with the control group, the escape lateneies[ (41.29 ± 20.33 )s vs (62.17 ± 20.36)s;(39.67 ± 19.60)s vs (63. 00 ± 22.16) s ] ,the times of crossing through the platform [ (6.13 ± 2.21 ) v s (2.04 ± 1.42) ; (5.41 ± 2.13 ) vs ( 3.12 ± 1.20 ) ] , the percentage of swimming within the platform quadrant of pool [ (51.14 ± 12.38 ) % vs ( 30.75 ± 14.03 ) % ; (47.11 ± 9.29 ) % vs ( 33.17 ± 10.45 ) % ] were different significantly in the treatment group and prevention group (P 〈 0. 05, P 〈 0.01 ). Conclusion DSS improved the capability of learning and memory of SAM-P8 mice.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2009年第3期223-225,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金(30472248),广东省科技计划项目(2006835601007)