当归芍药散对SAM-P8小鼠空间学习记忆能力的影响

Effects of Danggui Shaoyao San on the learning and memory abilities of SAMP-8 mice

目的观察当归芍药散（DSS）对快速老化小鼠空间学习记忆能力的影响，为当归芍药散治疗阿尔采默病提供实验依据。方法选用雌性SAM—P8鼠36只，分成4组：预防组（3月龄）、预防对照组（3月龄）、治疗组（6月龄）、治疗对照组（6月龄），每组8只。治疗组以DSS（浓度为0．5g／ml）灌胃1个月，预防组自由饮用DSS（0．02％，V／V）4个月，对照组给予冷开水，给药结束后行Morris水迷宫测试。结果Morris水迷宫测试预防组、预防对照组、治疗组、治疗对照组的平均逃避潜伏期分别是（41．29±20．33）s、（62．17±20．36）s、（39．67±19．60）s、（63．00±22．16）s；穿越平台次数分别是（6．13±2．21）次、（2．04±1．42）次、（5．41±2．13）次、（3．12±1．20）次；平台象限游泳距离百分比分别是（51．14±12．38）％、（30．75±14．03）％、（47．11±9．29）％、（33．17±10．45）％。上述指标在预防组、治疗组与其相应对照组比较差异有显著性（P〈0．05）。结论DSS能明显改善老年性痴呆模型SAM—P8小鼠的空间学习记忆能力。

Objective To observe the effect of Danggui Shaoyao San on the learning and memory abilities of SAMP-8 mice, as well as provide scientific basis for its treatment of Alzheimer＇ s disease. Methods Female SAM-P8 mice were divided into treatment group（6 months, n = 8 ） , treatment control group（6 months, n= 8） ,prevention group（3 months, n=-8） , prevention control group（3 months） randomly. The mice in treatment group were administrated 0. 5ml DSS through stomach for 1 month. The mice in prevention group drank DSS in water（0. 02% , V/V） for 3 months, then were tested learning and memory abilities by Morris water maze. Results Compared with the control group, the escape lateneies[ （41.29 ± 20.33 ）s vs （62.17 ± 20.36）s;（39.67 ± 19.60）s vs （63. 00 ± 22.16） s ] ,the times of crossing through the platform [ （6.13 ± 2.21 ） v s （2.04 ± 1.42） ; （5.41 ± 2.13 ） vs （ 3.12 ± 1.20 ） ] , the percentage of swimming within the platform quadrant of pool [ （51.14 ± 12.38 ） % vs （ 30.75 ± 14.03 ） % ; （47.11 ± 9.29 ） % vs （ 33.17 ± 10.45 ） % ] were different significantly in the treatment group and prevention group （P 〈 0. 05, P 〈 0.01 ）. Conclusion DSS improved the capability of learning and memory of SAM-P8 mice.